D5025

Sigma

 

Deoxyribonuclease I from bovine pancreas

Type IV, lyophilized powder, Protein ≥80 %, ≥2,000 Kunitz units/mg protein

Synonym:Deoxyribonucleate 5′-oligonucleotido-hydrolase, DNase I
CAS Number:9003-98-9
Enzyme Commission (EC) Number:3.1.21.1   ( BRENDA | IUBMB )
EC Number:232-667-0
MDL number:MFCD00130918

Description

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Analysis NoteProtein determined by biuret.
ApplicationUsed for the removal of DNA from protein samples.
Preparation NoteDerived from New Zealand-sourced pancreas
Unit DefinitionOne Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate. [Mg2+] = 4.2 mM.
Physical formLyophilized powder containing calcium chloride

Properties

typeType IV
formlyophilized powder
mol wtmol wt ~31 kDa
purified bychromatography
compositionProtein, ≥80%
EQP levelPremium
foreign activityChymotrypsin ≤0.5%
 Protease ≤0.05%
 RNase ≤0.02%
storage temp.−20°C

Safety

Safety Statements22-24/25
WGK Germany3
RTECSRF0750000

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References

referenceCull, M., and McHenry, C.S., Preparation of extracts from prokaryotes. Meth. Enzymol. 182, 147-153, (1990)
 Molloy, M.P., et al., Proteomic analysis of the Escherichia coli outer membrane. Eur. J. Biochem. 267, 2871-2881, (2000)
 Moore, S. The Enzymes 3rd ed.,, (1981) 14, 281
 Laskowski, M. The Enzymes 3rd ed.,, (1971) 4, 289
 Lindberg, U. Biochemistry 6, 335, (1967)
MerckMerck 13,2923